The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. For the major nodes, the percentage (> 75%) of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown. The evolutionary history was inferred using the neighbour‐joining method. Phylogenetic analysis of choline dehydrogenase (BetA). S1 showing strain names and their corresponding accession numbers (Gene ID in IMG/JGI).įig. ChoX, SBP specific for choline TmoX, SBP specific for trimethylamine N‐oxide BetX, SBP specific for glycine betaine CaiX, SBP specific for carnitine.įig. The scale bar represents the number of substitutions per amino acid. The tree was aligned in mega 5.2 using the neighbour‐joining method using 500 replications for bootstrapping. Characterized SBPs, related to osmolyte SBPs, based on the structural analysis conducted by Berntsson and colleagues (2010) were used as an outgroup. Reference sequences from characterized SBPs were added to the alignment. Phylogenetic analysis of the substrate binding proteins (SBPs) affiliated with the putative choline ABC‐type transporter found in marine bacteria.
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